Overview

The Lab of Immune Regulation, Resolution & Tolerance at Singapore Immunology Network (SIgN) is seeking a motivated and interdisciplinary postdoctoral scientist to join our team in the field of autoimmunity. 

The successful candidate will lead and contribute to advancing our understanding of immune tolerance is affected in autoimmunity, namely in Systemic Lupus Erythematosus (SLE) and lupus nephritis (LN). In particular, the candidate will explore  myeloid and lymphocyte crosstalk during disease progression, and investigate regulatory T cell deficits in SLE utilizing pre-clinical mouse models, with the opportunity to expand their findings to human samples. 

Job Requirements

Key Responsibilities:

Investigate the dynamic changes in immune composition and cross-talk during disease progression from SLE to lupus nephritis

• Conduct in vivo mouse model experiments, including mouse breeding, handling, surgical procedures, end-point sample harvesting, ensuring compliance with regulatory and ethical requirements.
• Design, perform, optimise and analyse high parameter flow cytometry experiments across both mouse and human tissues. 
• Conduct in vitro wet lab experiments, such as fluorescence activated cell sorting (FACS), cell culture of primary immune cells, immunofluorescence staining, among others. 
• Handle and manage human clinical samples, ensuring compliance with regulatory and ethical requirements.
• Collaborate with others to set-up and analyse large sequencing datasets including TCR-sequencing and spatial transcriptomics.
• Analyse data using a combination of standard software including Flowjo (flow cytometry) and bioinformatics tools (R, Python). 

Team contribution, mentoring and collaboration

• Provide mentorship and guidance to students and junior researchers in the lab
• Engage and work closely with multidisciplinary team of collaborators across Singapore
• Work closely with the PI to align research priorities and meet milestones

Job Requirements:

• Ph.D. in Biomedical Sciences/Engineering, with an Immunology specialization strongly preferred.
• Strong understanding of immunology, with a key focus on autoimmunity. 
• Strong track record of peer-reviewed publications appropriate to the current career stage
• Experience in designing and executing experiments with a high degree of technical rigour. Familiarity with cell culture, primary immune cell isolation and flow cytometry is preferred. 
• Familiarity with next generation sequencing, especially for spatial transcriptomics and TCRseq+scRNAseq is a plus.
• Excellent problem-solving and critical thinking skills with the ability to work independently and collaboratively within a team environment. 
• Strong written and oral communication skills, with experience in scientific publication and presentations

Why join us:

• Be part of a young and dynamic new team advancing research into immune tolerance and resolution
• Gain experience working at the intersection of wet and dry lab with access to state-of-the-art facilities and technologies
• Perform rigorous science with strong translational capabilities