We are seeking a highly motivated Senior Postdoctoral Fellow to establish and execute human induced pluripotent stem cell (hiPSC)-based differentiation workflows for generating lineage-specific cell types, including cortical neurons, cardiomyocytes, and hepatocytes. The candidate will support large-scale cell generation, banking, and molecular profiling activities for downstream genomic and functional applications.

Key Responsibilities

• Maintain and expand human iPSC lines under feeder-free, chemically defined conditions.
• Develop, optimize, and perform directed differentiation of hiPSCs into:
  • Cortical neurons through neural induction and maturation workflows.
  • Cardiomyocytes using small molecule-based cardiac differentiation protocols.
  • Hepatocytes through stepwise definitive endoderm, hepatic specification, and maturation protocols.
• Perform lineage-specific cell enrichment (e.g., MACS/FACS/metabolic selection), characterization, and quality control using molecular and cellular assays.
• Generate, process, and cryopreserve iPSC-derived cell populations for biobanking and future applications.
• Prepare high-quality RNA samples from purified cell populations for transcriptomic analyses, including ONT direct RNA sequencing.
• Establish standardized SOPs and QC workflows to ensure reproducibility and scalability.

Requirements

• PhD in stem cell biology, developmental biology, regenerative medicine, bioengineering, or related fields.
• Strong hands-on experience in human iPSC culture and directed differentiation.
• Experience with iPSC-derived cardiomyocytes, neural cells, or hepatocyte differentiation is highly desirable.
• Proficiency in cell culture, flow cytometry, immunostaining, RNA preparation, and cell banking.
• Experience with automation platforms or scalable cell culture workflows is advantageous.
• Strong ability to independently manage projects and work in a multidisciplinary environment.